Table 3.

Quality control and lower limit of detection levels of the different UCP-LF CAA assay formats

Assay name CAA antigen assay	Biological matrixa	Dry/wet formatb	Single test QC (CAA pg/mL)c	Triplicate LLOD (CAA pg/mL)d
SCAA20	serum	wet	10	5
SCAA500	serum	wet	1	0·5
SCAA4000	serum	wet	0-3	0·15
SCAA20	serum	dry	30	15
SCAA500	serum	dry	3	1·5
SCAA4000	serum	dry	1	0·5
UCAA10	urine	wet	10	5
UCAA250	urine	wet	1	0·5
UCAA2000	urine	wet	0-1	0·05
UCAA7500	urine	wet	0-03	0·015
UCAA10	urine	dry	30	15
UCAA250	urine	dry	3	1·5
UCAA2000	urine	dry	0-3	0·15
UCAA7500	urine	dry	0-1	0·05

^aResults achieved in the saliva-based matrix are not included. The saliva-based assay is not fully explored and determined levels are still considered tentative; clinical saliva-based samples from Schistosoma infected individuals were not yet tested.

^bThe dry assay format is developed for use by third parties (allows convenient transport of dry reagents without a cold chain). The wet assay format is used at LUMC to achieve maximum sensitivity.

^cThe QC (quality control) values present the detection values that need to be achieved when testing normal human sera or urine spiked with AWA-TCA (containing 3% w/w CAA). The CAA-20 level is the most stringent; UCP conjugates that do not pass the CAA-20 QC will not be used for preparation of dry reagents.

^dThe LLOD (lower limit of detection) is the level achievable when testing under ideal laboratory conditions or when performing multiple (triplicate or more) experiments.