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. 2014 Dec 15;28(24):2699–2711. doi: 10.1101/gad.248526.114

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© 2014 Zhou et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 6. — Gene-proximal enhancers are not required for Sox2 transcription in ES cells. (A) SRR1, SRR2, and SRR18 were each deleted in F1 ES cells using two gRNAs. ΔSRR1/2/18^Cast was generated using gRNA2(5′) with gRNA18(3′) in a ΔSRR1^Cast clone. (B) Allele-specific RT-qPCR revealed that SRR1, SRR2, and SRR18 are all dispensable for Sox2 expression in ES cells. The Sox2 mRNA levels shown are relative to the levels in F1 ES cells. Error bars represent standard deviation between at least three technical replicates for each deleted clone.