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. 2014 Dec 15;28(24):2726–2738. doi: 10.1101/gad.246546.114

Figure 6.

Figure 6.

WRAP53β protects cells against accumulation of spontaneous DNA damage. (A) U2OS cells were treated with siControl or two different siWRAP53 oligonucleotides (siWRAP53#1 and siWRAP53#2) for 24 h, fixed, and immunostained for WRAP53β and γH2AX. (B) The percentage of nuclei in A containing >10 γH2AX foci was quantified in the 200 cells counted for each experiment. (C) After treating U2OS with siWRAP53#2 or siControl for 24 h or 48 h, DNA damage was assessed by the alkaline comet assay. The error bars depict the SEM. n = 3; (**) P < 0.01; (***) P < 0.001, as determined by Student’s t-test.