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Flowchart of tumor rechallenges. After 14 days of each treatment including surgical excision and cryoablation on the left side of the tumors induced by RENCA injection, the RENCA cells were reinjected on the right side of the BALB/c mice, which was free of tumor recurrence. To verify tumor specific immunity, for 20 mice treated with cryoablation and free of tumor recurrence, CT26 cells were injected in a similar manner. FACS, fluorescent-activated cell sorting.
