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. 2014 Nov 28;55(12):834–840. doi: 10.4111/kju.2014.55.12.834

FIG. 2.

FIG. 2

Result of the fluorescent-activated cell sorting (FACS) analysis of the cryoablation and surgical excision. (A) The CD3, CD4, CD8, and natural killer cell counts measured by the FACS assay were significantly increased in the cryoablation group compared to the surgical excision group. The CD19 cell count was higher in the surgical excision group, but this difference was not statistically significant. *p<0.05, by Mann-Whitney U test. (B) The cytotoxic activity of lymphocyte after cryoablation. Mice with RECNA tumors treated by either cryoablation or surgical excision were sacrificed and spleens were harvested for a cytotoxicity assay against RENCA cell line. The significantly increased cytotoxicity of cryoablation group, compared with surgical excision or tumor control group, was manifested at E:T ratio of 40:1, and maintained to 5:1. The cytotoxicity of surgical excision was statistically increased also compared to control group, from E:T ratio of 40:1 to 10:1 (*p<0.05, by Kruskal-Wallis test and Mann-Whitney U test compared cryoablation group to surgical excision group or control group. †p<0.05, by Mann-Whitney U test compared excision group to control group). E:T ratio, ratios of effecter to target cells.