Fig. 1.

Collagen-induced SDF-1α release and regulation of platelet aggregation by CXCR4. A –B, Washed platelets (2 × 10⁸/mL^− 1) were pre-treated with vehicle control or inhibitors; (A) 100 nM AMD3100 (CXCR4) and (B) 10 μg/mL^− 1 11G8 (CXCR7) or IgG₁ control in the presence of 10 μg/mL^− 1 IV.3 and monitored for platelet aggregation using a range of collagen concentrations. Representative aggregation traces (A–Bi) and quantified area under the curve analysis (A–Bii) are shown. Data are mean ± SEM, n = 6, *** P < 0.001 vs. vehicle, Two-way ANOVA with Bonferroni post-hoc analysis. C) Washed platelets (4 × 10⁸ mL^− 1) were stimulated with 2.5 μg/mL^− 1 collagen for indicated time points under aggregating conditions, with subsequent analysis of platelet releasate by SDF-1α ELISA. Data are mean ± SEM, n = 4, **P < 0.01 vs. basal, One-way ANOVA with Dunnett's multiple comparison test.