Fig. 3.

Collagen-induced thrombus formation requires CXCR4-dependent signalling. PPACK- (40 μM) and Heparin- (2 U/mL^− 1) anticoagulated whole blood labelled with 2 μM DiOC6 was pre-treated with vehicle control or 100 nM AMD3100 and perfused over a collagen-coated surface for 6 min at a constant shear of 1000 s^− 1. Samples were subsequently washed and fixed in modified HEPES-Tyrodes containing 4% paraformaldehyde. Representative confocal images at the collagen surface (z position = 0 μm) with 4 μm increments, as indicated, are shown along with quantitative analysis of platelet thrombus coverage (%) and volume (μm³) (Ai–iii). Data are mean ± SEM, n = 4, *P < 0.05 vs. vehicle, two-tailed paired Student's t-test.