Fig. 5.

Regulation of cAMP and Ca(2 +) signalling by SDF-1α. A, Washed platelets (4 × 10⁸/mL^− 1) were pre-treated with vehicle control or 100 nM AMD3100 and then stimulated with 0.7% EtoH (final conc. — carrier for PGE₁) or 1 μM PGE₁ in the absence or presence of 100 ng/mL^− 1 SDF-1α. Samples were lysed and analyzed for total cAMP levels (pmol/mL^− 1) by ELISA. Data are mean ± SEM, n = 4, **P < 0.01, ***P < 0.001, One-way ANOVA with Dunnett's multiple comparison test. B, Washed platelets (1 × 10⁸/mL^− 1) were loaded with Fura2-AM and stimulated with 100 ng/mL^− 1 SDF-1α alone (Bi) or co-stimulated with 2.5 μg/mL^− 1 collagen and 100 ng/mL^− 1 SDF-1α (or vehicle) in the absence or presence of 100 nM AMD3100 to monitor changes in intracellular calcium responses (Bii). Traces shown are representative of 4 independent experiments.