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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Cold Spring Harb Protoc. 2013 Apr 1;2013(4):298–304. doi: 10.1101/pdb.top073890

Figure 1.

Figure 1

Live-cell confocal microscopy during morphogenesis. (A) Mediolaterally intercalating mesoderm cells labeled with both rhodamine-dextran to highlight the cytoplasm and nucleus and negatively mark yolk granules and intracellular vesicles and mem-GFP to highlight the plasma membrane at cell boundaries. Mem-GFP appears twice as intense when two membranes fall within the confocal depth of focus or in a single set of pixels. (B) GFP expressing live mesoderm assembles fibronectin fibrils along the interface where mesoderm abuts agarose. Synthesized fibronectin fibrils are labeled with a Cy3-conjugated fibronectin mAb (4H2) in real time. (C) Paxillin-GFP expressing animal cap edge cells cultured on a fibronectin coated glass substrate. (D) Frames from a confocal time lapse sequences of utrophin-mCherry expressing mesendoderm cells cultured on a fibronectin coated substrate.