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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Cold Spring Harb Protoc. 2013 Apr 1;2013(4):298–304. doi: 10.1101/pdb.top073890

Figure 2.

Figure 2

Processing and Analyzing Images. (A) Two confocal z-sections of mem-GFP are collected from a marginal zone explant, color encoded, and combined into a single merged image. Membrane protrusions are visible at z =0 and cell boundary is clear at the 5 μm level. Merged z-levels encoded by different colors can show how mesoderm cell protrusions on the substrate are coordinated with shape changes in the cell body. (B) A single frame from a confocal time lapse of tau-GFP collected for 10 minutes at a 10 second interval. Average or maximum time projections of tau-GFP present the dynamics of microtubule within elongated mesoderm cells over the time course of the movie. (C) Frames from a time-lapse of mem-GFP expressing cells can be used to extract cell shapes (C′), designate ROIs, and follow the shape changes over time for individual cells (marked in red and blue in C′).