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. 2014 Dec 15;127(24):5179–5188. doi: 10.1242/jcs.149633

Fig. 3.

Fig. 3.

Podosome induction signaling facilitates MT-dependent KIF1C deposition in lamellae. (A) PDBu facilitates the accumulation of KIF1C–GFP (green) in the cell periphery. Frames from a single-plane confocal image sequence at 30 seconds (left) and 24 minutes (right) of PDBu treatment. Pseudo-colored maps of KIF1C intensity [purple (low) to white (high)] at the indicated areas (white brackets) are shown to the upper right. See supplementary material Movie 1. (B) Deposition of KIF1C–GFP (green) in cell lamellae before (left) and after (right) a 90-minute PDBu treatment. See supplementary material Movie 2. (C) Fold increase in KIF1C–GFP intensity in cell lamellae in live cells with or without PDBu treatment, based on data as shown in B. Data show the mean+s.e.m. (N = 6–12); *P<0.01 (Student's unpaired two-tailed t-test). (D–G) Immunostained KIF1C (red, arrows) at the cell periphery. KIF1C modestly localizes to the lamellae of untreated cells (D), and accumulates in cell lamellae after a 40-minute PDBu treatment (E). No KIF1C accumulations are found in nocodazole (noc)-treated (F) and nocodazole-pretreated plus PDBu-treated (G) cells. The images show maximal intensity projections of confocal stacks. Arrows show KIF1C accumulations; dashed lines indicate cell outlines. (H) The percentage of the area in cell lamellae taken up by KIF1C accumulation, based on data as shown in D–G. Data show the mean+s.e.m. (N = 10); *P<0.001 (Student's unpaired two-tailed t-test).