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. 2014 Dec 15;127(24):5204–5217. doi: 10.1242/jcs.150961

Fig. 7.

Fig. 7.

Catabolic pathways in the gastrocnemius muscles of Rpt3−/− mice. (A) Immunoblotting analysis for the detection of muscle atrophy-related signaling pathway components. (B) Quantification of the data from A. The amount of phosphorylated p38 (pp38) was also increased in Rpt3−/− mice. No apparent differences were observed in the levels of Akt and phosphorylated (p)Akt. Foxo3a, p50, and myostatin were increased. Phosphorylated (p)Foxo3a, p70S6K1, phosphorylated (p)S6, S6, phosphorylated (p)4EBP1, 4EBP1 and GAPDH were also present. The ratios of phosphorylated S6∶S6 and phosphorylated 4EBP1∶4EBP1 were not altered, whereas the total amount of phosphorylated 4EBP1 was increased. Data show the mean+s.e.m.; *P<0.05 (Student's t-test). (C) Proteins related to RNA metabolism accumulated in myofibers. Expression of the FUS/TLS, VCP and TDP-43 proteins was increased in Rpt3−/− mice, especially in the insoluble fractions. (D) Immunohistochemistry of TDP-43, FUS/TLS and VCP revealed that the amount of these markers localizing within myonuclei in Rpt3−/− mice was increased. TOTO3, nuclei; OVL, overlay. Scale bars: 20 µm (upper panel), 50 µm (middle and lower panels).