Fig. 1.

Syt7 is expressed in isolated ATII cells and localised on the lamellar body membrane. (A) Real-time RT-PCR analysis of synaptotagmin transcripts in freshly isolated ATII cells from rat. Data are expressed as the relative expression compared to housekeeping gene Hmbs. Values are mean±s.e.m. from three individual cell isolations and are represented as mean±s.e.m. (B) Western blot of Syt7 from freshly isolated ATII cells and ATII cells 48 h after isolation confirms expression of Syt7 is not altered in cultured cells. Ponceau staining of blots (left) was used to control for equal loading of lanes. (C) Syt7 (red) is primarily localised on lamellar body membranes as detected by indirect immunofluorescence and confirmed by colocalisation with P180 lamellar body protein (green, ABCa3). Scale bar: 10 µm.