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. 2014 Dec 15;127(24):5261–5272. doi: 10.1242/jcs.156778

Fig. 2.

Fig. 2.

Slower keratinocyte proliferation, reduced apoptosis and dysregulation of Rb phosphorylation in the Epfn−/− epidermis. (A) PCNA (a,b), Ki67 (c,d) and BrdU (e,f) staining in the P7 epidermis, and TUNEL staining (g,h) in the P3 epidermis. Scale bars: 100 µm. (B) Relative numbers of PCNA-, Ki67-, BrdU- and TUNEL-positive cells in Epfn+/− and Epfn−/− skin. Data show the mean+s.e.m. (three independent experiments); **P<0.01. (C) Proliferation of primary keratinocytes from newborn Epfn+/− and Epfn−/− mice. Data show the mean+s.e.m. (three independent experiments); *P<0.05. (D) Flow cytometric analysis of cell cycle progression in primary keratinocytes from newborn Epfn+/− and Epfn−/− mice. (E) Expression of cell cycle regulators as revealed by western blotting. Reduced Rb phosphorylation (phos-pRb) and expression of cell cycle regulators in primary keratinocytes from newborn Epfn+/− and Epfn−/− mice. Upper arrowhead and asterisk, phos-Rb; lower arrowhead and asterisk, Rb protein.