Table 1.
Optical properties of NIR fluorophores in 100% serum supplemented with 50 mM HEPES (pH 7.4).
| Optical Property | MB | T700-H | T700-F | ZW800-1 | ZW800-3C | ESNF31 |
|---|---|---|---|---|---|---|
| FLARE Imaging Channel | 700 nm NIR (Channel #1) | 800 nm NIR (Channel #2) | ||||
| Extinction Coefficient (M-1cm-1) | 49,500 | 147,000 | 123,000 | 249,000 | 309,000 | 135,000 |
| Absorbance Maximum (nm) | 665 | 648 | 651 | 772 | 774 | 803 |
| Emission Maximum (nm) | 688 | 670 | 665 | 788 | 789 | 814 |
| Quantum Yield (%) | 9.6 | 30.4 | 29.9 | 15.1 | 16.1 | 10.8 |
| Molecular Brightness (M-1cm-1) | 4,752 | 44,688 | 36,777 | 37,599 | 49,749 | 14,580 |
In silico calculations of logD at pH 7.4 and total polar surface area were calculated using Marvin and JChem calculator plugins (ChemAxon, Budapest, Hungary).