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. 2015 Jan 1;5(1):12–22. doi: 10.7150/thno.8799

Figure 5.

Figure 5

Impact of kinase inhibitors on the phosphorylation levels of histone H3 and on the TMZ+Irrad-induced cell death. (A) Design of the experiments. (B-C-D) PCTC-GBMs presenting a high level of pH3 were treated with adequate kinase inhibitors. The effects of these treatments were estimated by monitoring the pH3 level by ELISA. Cell death is next estimated by using a Trypan Blue Stain 0.4%, and the Countess® Automated Cell Counter (Life Technology, France) after the TMZ+Irrad treatment (Temozomide+Irradiation treatment).