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. 2015 Jan 1;5(1):43–61. doi: 10.7150/thno.10350

Figure 2.

Figure 2

Detection of anti-ScFvEGFR antibody in mouse serum samples using ELISA. A. The level of anti-ScFvEGFR antibody in the serially diluted serum samples of tumor bearing mice following two i.v. deliveries of 200 pmol of ScFvEGFR-IONPs or equivalent unconjugated ScFvEGFR (50 µg). The level of mouse IgG antibody was measured. B. ELISA of mouse serum samples obtained from negative control IONP or PEG-IONP injected tumor bearing mice. C. Detection of the production of ligand specific IgG or IgM antibodies. Three injections of ScFvEGFR-IONPs activated high levels of both IgG and IgM ligand specific antibodies. Mice injected with PEG modified ScFvEGFR-PEG-IONPs had significantly higher level of IgG ligand specific antibody, but not IgM antibody, than ScFvEGFR-IONP injected mice. n=3 mice for all bar figures.