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. 2015 Jan 1;5(1):43–61. doi: 10.7150/thno.10350

Figure 3.

Figure 3

Detection of anti-mATF antibody in mouse serum samples using ELISA. Anti-mATF antibodies were induced in both tumor-bearing and normal Balb/c mice after receiving four i.v. delivery of 200 pmol of mATF-IONPs. B and C. Activation of anti-mATF antibody in tumor-bearing mice that received 50 µg of mATF peptides, 200 pmol of mATF-IONP, or 200 pmol of mATF-PEG-IONP. D. Specific activation of anti-targeting ligand antibodies. Serum samples obtained from ScFvEGFR-IONP or mATF-IONP injected mice were added to mATF and ScFvEGFR coated plates. ELISA results showed that there was no cross reactivity between anti-mATF antibody and ScFvEGFR antibody produced in the mice. The level of mouse IgG antibody was measured. The bar figures show the mean O.D. 450 nm values of each serial dilution of the mouse serum (n=3 mice).