Figure 4.

Uptake of targeting ligand conjugated IONPs without or with PEG modification. A. Differential uptake of targeting ligands conjugated IONP or PEG-IONPs by mouse macrophages detected by Prussian blue staining. The RAW 264.7 mouse macrophage cells or primary mouse macrophages with the nanoparticles were stained blue. Cells were lysed and the amount of iron was measured using a spectrometer at O.D. 700 nm. B. Uptake of the targeting ligand-conjugated IONP or PEG-IONP by mouse dendritic cells. The same experimental conditions were used as the macrophage study.