Figure 7.

Detection of ligand specific antibody producing B cells in mouse spleens using ELISPOT assay. Balb/c mice bearing 4T1 mouse mammary tumors received tail vein injections of 200 pmol IONP, ScFvEGFR-IONP or ScFvEGFR-PEG-IONP, without or with carrying Dox, once per week for three weeks. The mice were then sacrificed and spleens collected for ELISPOT assay. A. Images of antibody-producing B cell spots (brown) in wells incubated with spleen cells from the mice. An ELISPOT reader was used to take the image. The number of brown colored dots represented the number of antibody producing B cells. M1, M2 and M3 were spleen cells from three mice in each group. B. Quantification of the amount of ScFvEGFR specific IgG or IgM producing B cells in mouse spleen. A total of 3x10⁵ of spleen cells were added in each well for detection of targeting ligand specific antibody. The number was the mean of three mice in each group. Ab: antibody.