Figure 1.

Schematic of ligation-dependent PCR and CL detection. Step 1, unique probes (hybridization sequences with universal primers at the end) competitively hybridized to the target sequence. Step 2, DNA ligase-65 enzyme was used to join the probe pairs. Step 3, the ligation products were amplified by PCR with universal primers and dUTP, and extended PCR product oligonucleotides were represented by a broken line. Step 4, the amplification product was hybridized with MNPs-probe and separated specifically for CL detection.