Table 1.
Sequence of oligonucleotides probes and primers used.
| Probes | Sizea | Oligonucleotide Sequencesb | |
|---|---|---|---|
| Reference gene | GAPDH-R | 122 nt | 5'-gggttccctaagggttggaCAGGAGCGAACGAGATACTCCTGAGGTGGCATAGTGGGGTGGTGAATAC-3' |
| GAPDH-L | 5'-PHO-CATGTACAAAGCTTGTGCCCAGACTGTGAATtctagattggatcttgctggcac-3' | ||
| X- chromosome | RSK4-R | 112 nt | 5'-gggttccctaagggttggaCCAAGCGAATGCAACGTATCTTTACCCCGACAGCATTCCAATGA-3' |
| RSK4-L | 5'-PHO-GTTGGTTAAAGTATGACCACTCACAGtctagattggatcttgctggcac-3' | ||
| PCDH19-R | 114 nt | 5'-gggttccctaagggttggaTAGTCCGGACCTGCTGTTATGTAGAGAACCTCTTGCACAGAAAGAC-3' | |
| PCDH19-L | 5'-PHO-CAGTCCTAACCTTTCAAATGATGCCTtctagattggatcttgctggcac-3' | ||
| Y- chromosome | SRY-R | 110 nt | 5'-gggttccctaagggttggaCCAAGCGAATGCAACGTATCCTGGTAGAAGTGAGTTTTGGATAGTAAAATAAGTTTC-3' |
| SRY-L | 5'-PHO-GAACTCTGGCACCTTTCAATTTTGTCGtctagattggatcttgctggcac-3' | ||
| DAZ1-R | 125 nt | 5'-gggttccctaagggttggaTAGTCCGGACCTGCTGTTATTCCACGCCCAACCATAGCACTTGACC-3' | |
| DAZ1-L | 5'-PHO-GAGAATAATACGTTCTTCATAAATCAGTCAGTCACTTtctagattggatcttgctggcac-3' | ||
| 21- chromosome | DSCR3-R | 111 nt | 5'-gggttccctaagggttggaATACCCAGAACGACTATGCGCTGGTGGCAAAAGCAGGTAGTTTC-3' |
| DSCR3-L | 5'-GAGAGATGAGCACAACCCAGAGAAGtctagattggatcttgctggcac-3' | ||
| DSCR4-R | 112 nt | 5'-gggttccctaagggttggaCTACAGCACCTCAGGAGAGTGCCTTCCAGGTAGCTGCTCATAAG-3' | |
| DSCR4-L | 5'-PHO-CCCTTTCTAGAGAGTGATTGGGACTAtctagattggatcttgctggcac-3' | ||
| Universal primers | Forward | 23 nt | 5'-GTGCCAGCAAGATCCAATCTAGA-3' |
| Reverse | 19 nt | 5'-GGGTTCCCTAAGGGTTGGA-3' |
a Amplification products length.
b The lower-case letters indicate PCR primer sequences, the capital letters indicate DNA hybridization sequences, the underlined letters indicate tag sequences; the 5' end of the right probe is phosphorylated (PHO) to allow ligation to the 3' end of the left probe.