Figure 4.

Monitoring of therapeutic effects of xenografted tumors expressing hNIS or opt-hNIS. (A) Therapeutic effects of codon optimization were monitored by bioluminescence imaging. Tumors expressing hNIS and opt-hNIS were grown for 2 weeks in nude mice. After ¹³¹I therapy, both hNIS- and opt-hNIS-expressing tumor sizes were reduced, but the size reduction of the opt-hNIS-expressing tumor (right flank) was more prominent than that of the hNIS-expressing tumor (left flank). (B) Sizes of hNIS-expressing and opt-hNIS-expressing tumors after ¹³¹I challenge were measured with calipers. At 20 d after radioiodine therapy, tumor sizes relatively decreased by 0.57- and 0.27-fold for hNIS- and opt-hNIS-expressing tumors, respectively, compared to the tumor sizes before therapy (n=3, P<0.05).