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Design of the crossover CEUS study.
| Day | Dogs | Microbubble injections |
|---|---|---|
| Week 1, day 1 | 1, 2, 3 | Left: unloaded microbubbles Right: mRNA-loaded microbubbles |
| Week 1, day 2 | 4, 5, 6 | Left: mRNA-loaded microbubbles Right: unloaded microbubbles |
| 2 week wash-out | ||
| Week 3, day 1 | 1, 2, 3 | Left: mRNA-loaded microbubbles Right: unloaded microbubbles |
| Week 3, day 2 | 4, 5, 6 | Left: unloaded microbubbles Right: mRNA-loaded microbubbles |
