Fig. 1. cub and Mcub are mutations at the Rhbdf2 and Areg loci, respectively.

(A) Coverage depth across the Rhbdf2 locus in cub/cub mice, and predicted translational consequences of the cub deletion on iRhom2 protein. (B) Filtering pipeline for variants detected after whole-genome sequencing of cub/cub;Mcub/Mcub DNA. (C) Identification and predicted consequences of the Areg^Mcub variant. (D) The bald phenotype of an Rhbdf2^cub/Rhbdf2^cub mouse (Johnson et al., 2003). (E) Rhbdf2^KO/Rhbdf2^KO mouse has a WT coat (Adrain et al., 2012; McIlwain et al., 2012). (F) Rhbdf2^cub is recessive: Rhbdf2^cub/+ mouse has a WT coat (Johnson et al., 2003). (G) Rhbdf2^cub/Rhbdf2^KO mouse has sparse hair (Hosur et al., 2014). Mice in panels D–G are on a C57BL/6J background, except for Rhbdf2^cub/Rhbdf2^KO, which is on a mixed C57BL/6J/129 background.