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. 2014 Nov 13;3(12):1151–1157. doi: 10.1242/bio.201410116

Fig. 3. TACE maturation is not promoted by the cub mutant of iRhom2.

Fig. 3.

(A) Western blot of TACE from lysates and ConA enrichment preparations from iRhom1/2 double knock-out MEFs transduced with GFP, iRhom2-HA or the cub mutant of iRhom2-HA. Levels of the transferrin receptor (TfR) were used as a loading control. Note that only iRhom2-HA promotes the maturation of TACE. In the blot for TACE, immature TACE is indicated with an empty arrowhead (i), mature TACE with a filled arrowhead (m). In the blot for HA, full-length iRhom2-HA is indicated with a filled arrowhead, and full-length cub-HA is indicated with an empty arrowhead. Asterisks (*) indicate sub-fragments of iRhom2-HA or cub-HA regularly observed on western blot. (B) Densitometric quantification of the ability of iRhom2-HA and cub-HA to promote the maturation of TACE in iRhom1/2 double knockout MEFs. Data represent the ratio of mature to immature TACE, relative to iRhom1/2 double knockout MEFs transduced with GFP (in which TACE maturation is blocked). n = 4 individual experiments. Error bars represent standard deviation.