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. 2014 Nov 13;3(12):1151–1157. doi: 10.1242/bio.201410116

Fig. 4. The cub mutation impairs iRhom2 binding to TACE.

Fig. 4.

(A) Western blot analysis of HA-immunoprecipitates from HEK293 cells transduced with empty vector, iRhom2-HA or cub-HA and probed for binding to endogenous TACE. Cells were treated with or without 200 nM PMA, to induce shedding for 15 mins, but this had no effect on binding to TACE. (B) Western blot analysis of ConA concentrated samples from HEK293 cells transduced with empty vector, iRhom2-HA or cub-HA and probed for endogenous TACE and beta-actin. In the blots for TACE, immature TACE is indicated with an empty arrowhead (i), mature TACE with a filled arrowhead (m). In the blot for HA, full-length iRhom2-HA is indicated with a filled arrowhead, and full-length cub-HA is indicated with an empty arrowhead. Asterisks (*) indicate sub-fragments of iRhom2-HA or cub-HA regularly observed by western blot.