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. 2014 Nov 13;3(12):1173–1182. doi: 10.1242/bio.20149845

Fig. 3. Analysis of the lateral diffusion of CX3CL1 and CX3CR1 in various cell types.

Fig. 3.

(A) Kinetics of the fluorescence recovery after photobleaching of a 5-µm-diameter bleached area at the surface of COS7 cells expressing CX3CL1-EYFP (filled squares) or CX3CR1-EYFP (empty squares) chimeras. (B) Dependence as a function of the surface of the bleached area of the FRAP characteristic time of CX3CL1 expressed in COS7 cells either as an EYFP chimera (filled squares) or bound to FITC-stained specific antibody (empty squares), of FITC-Ab bound CX3CL1 in HUVEC cells (filled circles), and of CX3CR1-EYFP expressed in COS cells (filled triangles). (C) The diffusion rate of CX3CL1-EYFP was measured at the surface of COS7 cells expressing different CX3CL1-EYFP levels as the membrane fluorescence level measured by confocal microscopy. The black square represents the data obtained using COS7 transfected under the conditions that were classically used throughout this study. (D) The diffusion rates of CX3CL1-EYFP (filled bars) and of CX3CR1-EYFP (empty bars) were measured in different cell lines as indicated.