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. 2014 Nov 28;3(12):1228–1235. doi: 10.1242/bio.201410132

Fig. 1. Contrary to HUVEC, EA.Hy926 cell line does not align in response to shear stress.

Fig. 1.

(A) Cell surface expression of ICAM-1 on HUVEC and EA.hy926 exposed or not to shear stress of increasing strength for 18 h. Data are means ± SE (N = 5), *p<0.05, significantly different from static condition. (B) HUVEC and EA.hy926 cell line under static condition or exposed to fluid shear stress at 2.0 Pa for 5 h were stained by VE-cadherin. (C) The organization of the actin cytoskeleton of HUVEC and EA.hy926 monolayers under static conditions or after shear stress stimulation (2.0 Pa for 18 h). White arrow: indicates the direction of flow. (D) Alignment of cells was determined as cell orientation (0° indicates perfect alignment with flow direction; 45° indicates random orientation). (E) Cell elongation was established as the elliptical form factor (increasing levels of elliptical form factor indicates elongation). Data are means ± SE (N = 5), *p<0.05. (F) Confluent EA.hy926 monolayers were maintained under static conditions or exposed to shear stress of 3.7 Pa for 48 h. White arrow indicates flow direction. The results shown are representative of three independent experiments. Scale bars: 10 µm (B,C), 100 µm (F).