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. 2014 Nov 28;3(12):1228–1235. doi: 10.1242/bio.201410132

Fig. 3. CCM complex inhibits β1 integrin-dependent morphological response of EC to fluid shear stress.

Fig. 3.

(A) Western blots of β1 integrin protein, ICAP-1, CCM1 and CCM2. Quantifications are means ± SE (N = 8). (B) Relative mRNA levels of β1 integrin, ICAP-1, CCM1 and CCM2 in HUVEC (set as 100%) and in EA.hy926 cell line. Black bars, HUVEC; white bars, EA.hy926 cell line. Data are means ± SE (N = 4) normalized using housekeeping genes ATP 50 and RPLO. (C) Staining of F-actin on CT- or CCM2-silenced EA.hy926 under static conditions or exposed to fluid shear stress at 2.0 Pa for 18 h. White arrow: indicates the direction of flow. (D) Silencing of CCM2 by siRNA in EA.hy926 analyzed by western blot. (E) Elongation of CT- or CCM2-silenced EA.hy926 under static conditions and cell orientation of CT- and CCM2-silenced EA.hy926 or HUVEC exposed to fluid shear stress at 2.0 Pa for 18 h. Data are means ± SE (N = 2), *p<0.05. Scale bar: 25 µm (C).