Fig. 4. CCM2 negatively regulates β1-integrin mediated cytoskeletal reinforcement and reorientation after shear stress stimulation.

HUVEC, EA.hy926 and CCM2-silenced EA.hy926 were exposed to fluid shear stress at 2.0 Pa for 18 h. Cells were stained by (A) paxillin and activated-state-specific β1-integrin antibody (Clone:9EG7) and by (B) F-actin and zyxin antibody. White arrow: indicates the direction of flow. Scale bars: 10 µm.