Fig. 6. Slow-cycling cells pause in G2 phase.

DNA content of cells that retained label and those that did not retain label was measured using DNA content of neurons as standard for 2N DNA content. The experiment was performed at 1 week of chase for interstitial cells and at 3.5 week of chase for epithelial cells. (A) The percentage of label-retaining cells and cells without label in different phases of cell cycle. (B) Cell cycle analysis of 1s/2s cells. (C) Cell cycle analysis of epithelial cells. Label-retaining cells of all lineages were predominantly present in the G2 phase. Data pooled from three independent experiments are shown.