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. 2014 Nov 21;3(12):1252–1261. doi: 10.1242/bio.201410041

Fig. 4. Expression of a Slmb-anti-GFP-DARPin fusion in Drosophila embryos degrades eYFP.

Fig. 4.

(A) Shown is an embryo with the following genotype: H2A-eYFP; en-GAL4, UAS-mCherryNLS; UAS-Slmb-3G86.32. Nuclei that express the Slmb-anti-GFP-DARPin in the engrailed pattern are also expressing unfused mCherry. It can be seen that red cells lost the eYFP signal. (B–B″) Close-up of another embryo showing the channel-specific signal of eYFP (B), mCherry (B′) and the overlay (B″). Note again that cells expressing mCherry have strongly reduced H2A-eYFP signal, indicating efficient eYFP degradation due to the expression of a Slmb-anti-GFP-Darpin. Scale bars are 20 µm.