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. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: J Immunol. 2014 Oct 13;193(10):5076–5087. doi: 10.4049/jimmunol.1400529

Figure 1. Coinfection with EF3030+PR8 leads to enhanced disease and mortality.

Figure 1

A. Overview of experimental design. Animals infected as shown in A (singly infected with PR8, EF3030 or coinfected) were monitored for disease (B) and mortality (C). Disease was quantified using an adapted version of Tate’s disease scoring criteria which used the following parameters; coat ruffling, labored breathing, hypoxia, dehydration, ataxia and lethargy, conjunctivitis, and coat hygiene. Animals euthanized because of disease state were excluded from the disease score calculation after removal. Dotted line indicates day of bacterial infection. Data are derived from 26 (PR8), 44 (PR8+EF3030), or 20 (EF3030) animals assessed across 3 experiments. A Kruskal-Wallis non-parametric ANOVA with a Dunn’s posttest (B) and a Fischer’s Exact test (C) were used to determine significance. **** p<0.0001, *** p<0.0002