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. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: J Immunol. 2014 Oct 13;193(10):5076–5087. doi: 10.4049/jimmunol.1400529

Figure 2. Coinfection with PR8 and EF3030 leads to significantly increased viral RNA 24 hours after coinfection and significantly increased bacterial burden in the lung through d11.

Figure 2

Animals were infected as described in figure 1. Lungs were harvested for viral and bacterial load analysis at the indicated times post influenza infection. A. Viral RNA was extracted from lung homogenates using a QIAamp Viral RNA Mini Kit. cDNA was synthesized from mRNA by reverse transcription and subjected to qRT-PCR using primer probes specific for PR8 HA to enumerate viral RNA copies per lung. B. A portion of the lung homogenate was serially diluted and plated on tryptose blood agar plates for bacterial quantification. Data are the average of 24–29 individually analyzed animals/infection condition assayed over three independent experiments. A two-tailed student’s t test was used to determine significance. * p<0.05, ** p<0.01, *** p<0.001**** p<0.0001