Skip to main content
. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: J Immunol. 2014 Oct 13;193(10):5076–5087. doi: 10.4049/jimmunol.1400529

Figure 8. The reduction in IFNγ production in NP-specific cells in the lungs of coinfected animals is correlated with decreased IFNγ mRNA.

Figure 8

On day 8 post influenza virus infection, CD8+tetramer+ and CD8+tetramer− lung cells from singly or coinfected animals were isolated by FACS sorting. The sorted populations were cultured in the presence of NP147-155 peptide for 5h to induce cytokine production. Following stimulation, mRNA was isolated and IFNγ, perforin, and granzyme B message quantified by qRT-PCR. Message levels were normalized to GAPDH. In all cases, the fold increase compared to the level of each mRNA present in tetramer negative CD8+ T cells from PR8 infected animals was calculated. Data are the average from two independent experiments assessing a total of 6 influenza or 7 coinfected mice. A two tailed student’s t test was used to determine significance. * p<0.05, ** p<0.01