Figure 3.
Patterns of somatic and germline chimerism in Chimera SB23. This bi-chimera was sacrificed 142 days after fusion of two oozooids, and one somatic sample and four testes were isolated and analyzed by AFLP as described in the methods. Bands unique to each genotype are illustrated (solid and open arrows). Somatic (S1) and testis (T1-T4) fingerprints are outlined. AFLP fingerprints of one of the four testis samples exactly match the somatic sample, while three testis samples are divergent, but match each other. As shown, each independent testis is derived from either one or the other genotype present in the chimera. Thus within the detection level of the AFLP technique (Fig. 2), individual testis are clonal and derived from a single genotype within the chimera.
