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. 2014 Dec 15;4:7478. doi: 10.1038/srep07478

Figure 2. Evaluation of three internal reference candidates in monoculture and syntrophic dual-culture.

Figure 2

CT is the qPCR quantification cycle, the fractional cycle number where fluorescence increases above the threshold. 13 cells from monoculture and 13 cells from syntrophic dual-culture were used to evaluate the consistency of the internal reference genes. The standard deviations (SD) across single cells were calculated using the OriginPro 8.0 software. Mean Ct values and SD values calculated using 13 single cells from each of the two conditions (i.e., mono- or dual-cultures) were shown above the plots.