Skip to main content
. 2014 Dec 15;4:7481. doi: 10.1038/srep07481

Figure 6. SIRT1 deficiency affects the expression of p53 and genes involved in self-renewal of SW620 cells.

Figure 6

(A) Quantitative real time PCR analysis of p53 mRNA levels in control SW620 cells and cells transduced with shRNAs or Scr-ShRNA. (B) Western blotting of p53 and GAPDH of control SW620 cells and cells transduced with shRNAs or Scr-ShRNA. (C) Quantitative western blotting analysis of p53 protein levels in control SW620 cells and cells transduced with shRNAs or Scr-ShRNA. (D) Quantitative real time PCR analysis of the mRNA levels of stemness-associated genes (Oct4, Nanog, Cripto, Tert, Lin28, Sox2, Smo and Bmi-1) in control SW620 cells and cells transduced with shRNAs or Scr-ShRNA. All data are representative of three independent experiments. Significance: *P<0.05, **P<0.01, compared with the controls.