Table 2. provides the list of UPR genes and the primer sequences for PCR array validation.
| UPR gene | Sequence | Product size (b.p) |
|---|---|---|
| EDEM3 |
F: 5′-GGAAGATGCATCCGAGTATTGTGG-3′
R: 5′-AGTCACAGCTTTCCCTATGGAAGG-3′ |
390 |
| SCAP |
F: 5′-CTGAGAGTGTTCCGTCTGGA-3′
R: 5′-CAGGTCCTGCTGAATGGAGT-3′ |
300 |
| SEL1L |
F: 5′-GTTCATGGGACATGGTACCTAAGC-3′
R: 5′-AGACAGTCGGCTAACCAACAAACC-3′ |
333 |
| ERN2 |
F: 5′-CGCTGTGGACATCTTCTCTG-3′
R: 5′-ACTCCTTCTCCAGCCAGTCA-3′ |
299 |
| HSPA5 |
F: 5′-GCTCTCTGGTGATCAAGATACAGG-3′
R: 5′-AAGGTGACTTCAATCTGTGGGACC-3′ |
291 |
| PFDN5 | F: 5′-GAGACTTCCTCTTCGTTAAGTCGG-3′ R: 5′-CTCTACATAGTACCCAGTTCCCAC-3′ | 313 |