Table 3. provides the list of ion transporters and the primer sequences for quantitative RT–PCR.
Ion transporter | Sequence | Product size (b.p) |
---|---|---|
Na/K/ATPase |
F - 5′- ATGTGCCCAGTGAACCGAAA −3′
R - 5′- TGAACGGGAAGGACATTTGG - 3′ |
253 |
NHE1 |
F – 5′- GACTACACACACGTGCGCACCCC-3′
R – 5′ – TCCAGGATGATGGGCGGCAGCAGGAAGAGGAA – 3′ |
233 |
pNBC |
F – 5′-CCAAGAAATCCAACCTTCGG-3′
R- 5′-GTCATTCAGACTGGAGGAAG-3′ |
134 |
MCT1 |
F-5′-TGACCATTGTGGAATGCTGT-3′
R: 5′-TTTCTGGTCCGGAGATTCTG-3′ |
281 |
MCT2 |
F: 5′- TGAAACTCTCATGGACCTCGT-3′
R: 5′- ACACGCTTGCTGCTACCAC-3′ |
182 |
MCT4 |
F: 5′-AAACCCTCCTGCCTCCACCAGA-3′
R: 5′-TGACGAAACAGCCGAAGAGCACG-3′ |
145 |
SLC4A11 |
F: 5′-TGCTCTATGGCCTCTTCCTC-3′
R: 5′-CCCTCCGGATGTAGTGTGTC-3′ |
123 |
β-actin | F: 5′-GCAAAGACCTGTACGCCAAC-3′ R: 5′-AGTACTTGCGCTCAGGAGGA-3′ | 144 |