Table 2. Virus inactivation assay.

Incubation time	Virus	Incubation condition	Viral titer* ± SEM **
0 h	HPV-16	Ethanol	3.2 × 106 ± 0.4 × 106
		25HC	3.4 × 106 ± 0.4 × 106
		27HC	3.3 × 106 ± 0.1 × 106
	HRoV	Ethanol	5.8 × 105 ± 0.7 × 105
		25HC	5.3 × 105 ± 0.4 × 105
		27HC	5.9 × 105 ± 0.7 × 105
	HRhV	Ethanol	1.5 × 105 ± 0.2 × 105
		25HC	2.1 × 105 ± 0.1 × 105
		27HC	2.3 × 105 ± 0.3 × 105
2 h	HPV-16	Ethanol	2.0 × 105 ± 0.1 × 105
		25HC	2.5 × 105 ± 0.1 × 105
		27HC	2.3 × 105 ± 0.2 × 105
	HRoV	Ethanol	4.5 × 105 ± 0.2 × 105
		25HC	4.2 × 105 ± 0.1 × 105
		27HC	4.9 × 105 ± 0.2 × 105
	HRhV	Ethanol	9.4 × 104 ± 0.6 × 104
		25HC	10.7 × 104 ± 0.7 × 104
		27HC	9.2 × 104 ± 0.9 × 104

*viral titer is expressed as FFU/ml for HPV-16 and HRoV and in PFU/ml for HRhV.

**SEM: standard error of the mean.