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. 2014 May 13;522(17):3795–3816. doi: 10.1002/cne.23622

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© 2014 Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Generation of medulla volume models. A: Arborizations of retinotopic afferents (green) within medulla columns in relation to DLG-immunostained medulla strata (magenta). A long-form R7 (arrowhead) defines the proximal boundary of M6. B: Identification of specific strata in the distal medulla (M1–M6) based on differences in DLG-immunostaining intensity (left), using specific columnar neurons as references (right). C: Segmentation of the M6 stratum (magenta) and medulla (yellow) from the DLG-immunostained optic lobe (gray). D: Volume rendering of the M6 stratum (magenta) within the medulla (gray). E: Spatial relationship of M6 (magenta), medulla (yellow), lobula, and lobula plate (gray) volume models. F: The M6 volume model provided by the standard model fly brain. Med, medulla; Lob, lobula; Lop, lobula plate; MB, mushroom body; AL, antennal lobe. Scale bars = 10 μm.