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. 2014 May 13;522(17):3795–3816. doi: 10.1002/cne.23622

Figure 8.

Figure 8

Neurotransmitters of M6-LNs in 30A-GAL4. A: Cell bodies at the dorsal (A1), medial (A2), and ventral (A3) LCBR are labeled by GFP (green) and immunostained by three different antibodies (magenta). Dorsal and ventral M6-LN cell bodies are anti-GABA immunopositive (solid arrowheads) but anti-DVGLUT immunonegative (open arrowheads). Medial M6-LN cell bodies are anti-DVGLUT immunopositive (solid arrowheads). All M6-LN cell bodies are anti-5HT immunonegative (open arrowheads). B: MARCM clones of five nondirectional M6-LNs. Syt::HA-positive terminals (magenta) and Syt::HA-negative terminals (green) are mixed. Insets: The nondirectional M6-LNs (green) in B are anti-GABA immunopositive (magenta). C: Anti-DVGLUT immunostaining at posterior M6. A representative optical section (1 μm in thickness) shows colocalization (solid arrowheads) between M6-LN boutons (green) and anti-DVGLUT-immunopositive signals (magenta). D: The remaining M6-LN boutons after Cha-GAL80 inhibition are anti-DVGLUT immunonegative (open arrowheads). E: Cha-GAL80 inhibition eliminates all anti-DVGLUT-immunopositive cell bodies but not anti-GABA-immunopositive cell bodies. Cell bodies at dorsal (E1), medial (E2), and ventral (E3) LCBR are labeled by GFP (green) and immunostained by two different antibodies (magenta). Genotypes: 1) UAS-mCD8::GFP;+/30A-GAL4 (B,C) and 2) FRT19A,UAS-mCD8::GFP;+/30A-GAL4;+/Cha-GAL80 (D,E). Scale bars = 10 μm.