Table 1.

Primary Antibodies Used for Immunohistochemistry

Antibody	Immunogen	Source	Dilution	Specificity
Mouse anti-DLG	DLG PDZ2 domain fused to GST	DSHB, Iowa City, IA	1:50	Drosophila lamina and central brain (Hamanaka and Meinertzhagen, 2010)
				Drosophila larva NMJ (Lahey et al., 1994; Parnas et al., 2001)
Rabbit anti-HA	YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH	Abcam, ab9110	1:500	Transgenically expressed in Drosophila PN axons (Jefferis et al., 2007)
Rabbit anti-5HT	Serotonin creatinine sulfate complex conjugated to BSA	Sigma, S5545	1:500	Colocalized immunoreactivity with tryptophan hydroxylase (Park et al., 2006; Huser et al., 2012)
Rabbit anti-GABA	γ-Aminobutyric acid (GABA) conjugated to BSA	Sigma, A2052	1:500	Drosophila lamina C2 neurons (Enell et al., 2007; Kolodziejczyk et al., 2008)
Rabbit anti-DVGLUT	The peptide CQMPSYDPQGYQQQ encoding amino acids 620–632 of DVGLUT	A. DiAntonio (Washington University School of Medicine)	1:5,000	Transgenically expressed in Drosophila embryo (Daniels et al., 2004)
				Western blotting characterization (Daniels et al., 2006)