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. 2014 Jan 1;522(13):2928–2950. doi: 10.1002/cne.23558

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© 2014 Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Glial cell morphology following ATP-induced photoreceptor degeneration. Sections of retinae from saline- and ATP-treated eyes were labeled for Müller glial cells (GS, green), astrocytes and Müller cell gliosis (GFAP, red), and cell nuclei (DAPI, blue) at 3 months (A: saline; B,C: ATP) and 6 months (D: saline; E,F: ATP) post-injection. For each time point, two example regions of ATP-treated retinae are presented. Asterisk in E indicates holes representing fluid-filled vacuoles and/or RPE cell migration into the retina. Arrowhead in F indicate extreme remodeling events of GFAP/GS-positive Müller cell processes displaced into the choroid. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars = 20 μm.