Table 1.
Primary Antibodies Used for Immunohistochemical Analysis
| Cell type/target | Antibody/lectin | Dilution | Source | Immunogen |
|---|---|---|---|---|
| Cones | Fluorescein-labeled peanut agglutinin (PNA) from Arachis hypogaea (peanuts) | 1:250 | Catalog No. FL-1071; Vector Laboratories, Burlingame, CA | NA |
| Photoreceptor and bipolar cell terminals | Guinea pig antivesicular glutamate transporter (VGLUT1), polyclonal | 1:1,000 | Catalog No. AB 5905; Millipore, Merck, VIC, Australia | C-terminal peptide of rat VGLUT1, GATHSTVQPPRPPPPVRDY |
| Horizontal cells | Mouse anticalbindin D28k, monoclonal | 1:4,000 | Catalog No. AB 300; Swant, Bellinzona, Switzerland | Purified bovine kidney calbindin-D28K |
| Rod bipolar cells | Mouse antiprotein kinase Cα (PKC), monoclonal (MC5) | 1:4,000 | Catalog No. P5704; Sigma-Aldrich, NSW, Australia | Peptide amino acids 296–317 of PKC |
| Amacrine and ganglion cells | Mouse anticalretinin, monoclonal | 1:1,000 | Catalog No. 63B; Swant, Bellinzona, Switzerland | Recombinant human calretinin-22k |
| Blood vessels | Lectin from Bandeiraea simplicifolia BS-I isolectin B4 FITC | 1:75 | Catalog No. L2895; Sigma,-Aldrich, NSW, Australia | N/A |
| Microglia | Rabbit anti-ionized calcium-binding adaptor molecule 1 (IbA1), polyclonal | 1:1,500 | Catalog No. 019–19741; Wako Pure Chemical Industries, Richmond, VA, USA | Synthetic peptide corresponding to the C-terminus of IbA1: PTGPPAKKAISELP |
| Müller cells | Mouse antiglutamine synthetase (GS), monoclonal (GS-6) | 1:1,000 | Catalog No. MAB302; Millipore, Merck, VIC, Australia | Glutamine synthetase purified from sheep brain |
| Astrocytes and gliotic Müller cells | Rabbit antiglial fibrillary acid protein (GFAP), polyclonal | 1:20,000 | Catalog No. Z0334;Dako,Carpinteria, CA, USA | Bovine spinal cord GFAP |
| Cells in G1–S transition | Rabbit anticyclin-D1, monoclonal (SP4) | 1:2 | Catalog No. AB21699; Abcam, Sapphire Bioscience, NSW, Australia | A synthetic peptide from the C-terminus of human cyclin D1 (amino acids 250–295, Uniprot seq: P24385) |
| All cycling cells, those in G1, S, G2, and M | Rabbit anti-1,098-bp Ki67 motif-containing cDNA fragment, monoclonal (SP6) | 1:1,000 | Catalog No. AB16667; Abcam, Saphire Bioscience, NSW, Australia | A synthetic peptide from the C-terminus of human Ki-67 (amino acids 2319–2323, FKELF, within amino acids 2300–2400, Uniprot seq: P46013) |
1
All antibodies listed were used as tissue markers and were found to label cellular morphology and distribution as demonstrated in previous publications.