Table 2.
Calculated and Measured Mono-Isotopic Masses [M+H]+
| mean | abundance [%] | |||||
|---|---|---|---|---|---|---|
| Peptides | sequence | calculated | measured | mean deviation | male (n=20) | female (n=20) |
| A-type allatostatins | ||||||
| AST-A 4 | RVYDFGLa | 868,4676 | 868,4621 | 0,0444 | 45 | 40 |
| AST-A 5 | LPNRYNFGLa | 1092,5949 | 1092,6023 | 0,0589 | 70 | 75 |
| AST-A 3 | ASAYRYHFGLa | 1183,6007 | 1183,6038 | 0,0565 | 70 | 70 |
| AST-A-PP | RYIIEDVPGA-OH | 1132,5997 | 1132,5747 | 0,0669 | 15 | 20 |
| C-type allatostatins | ||||||
| AST-C | QIRYRQCYFNPISCF-OH | 1935,9154 | 1935,9050 | 0,1660 | 10 | 5 |
| AST-C | pQIRYRQCYFNPISCF-OH | 1918,9100 | 1918,9164 | 5 | 0 | |
| Allatotropin | ||||||
| AT | APFRNSEMMTARGFa | 1613,7675 | 1613,7534 | 0,0749 | 70 | 55 |
| Slfamide | ||||||
| SIFamide | GYRKPPFNGSIFa | 1381,7375 | 1381,7448 | 0,0657 | 100 | 100 |
| FMRFamides | ||||||
| FMRFa-3 | AGQGFMRFa | 912,4514 | 912,4546 | 0,0393 | 20 | 10 |
| FMRFa-7 | GSGNLMRFa | 880,4463 | 880,4472 | 0,0414 | 15 | 5 |
| Myoinhibitory peptides | ||||||
| Mip 3 | VNAGPAQWNKFRGSWa | 1716,8723 | 1716,8640 | 0,0900 | 15 | 10 |
| short neuropeptide F | ||||||
| sNPF-1 (4–11) | SPSLRLRFa | 974,5894 | 974,5958 | 0,0457 | 100 | 100 |
| sNPF-2 (4–11) (x2) | APQLRLRFa | 999,6210 | 999,6275 | 0,0472 | 100 | 100 |
| sNPF-3 | APSQRLRWa | 1012,5805 | 1012,5877 | 0,0473 | 100 | 100 |
| sNPF-1 | AVRSPSLRLRFa | 1300,7966 | 1300,8048 | 0,0722 | 15 | 20 |
| Neuropeptide-like precursor 1 | ||||||
| NPLP-4 | NLASARASGYMLNa | 1366,6901 | 1366,6994 | 0,0659 | 100 | 100 |
| NPLP-5 | NIASLARKYELPa | 1373,7905 | 1373,7980 | 0,0660 | 100 | 100 |
| NPLP-1 | SYRSLLRDGATFa | 1384,7337 | 1384,7599 | 0,0663 | 60 | 65 |
| NPLP-6 | NIQSLLRTGMLPSIAP-OH | 1710,9576 | 1710,8202 | 0,0777 | 90 | 95 |
| ext. NPLP-6 | NIQSLLRTGMLPSIAPK-OH | 1839,0521 | 1839,0482 | 0,0732 | 95 | 85 |
| NPLP-2 | NLGSLARAGLLRTPSTDYL-OH | 2018,1034 | 2018,0983 | 0,0625 | 75 | 65 |
| NPLP-7 | NMQSLARDNSLPHFAGAAAQES-OH | 2315,0838 | 2315,1143 | 0,0787 | 70 | 55 |
| sulfakinin | ||||||
| SK-1 | FDDYGHMRFa | 1186,5104 | 1186,5011 | 0,0628 | 25 | 45 |
| SK-2 | GGGGEGEQFDDYGHMRFa | 1857,7615 | 1857,7446 | 0,0792 | 25 | 20 |
| Tachykinin related peptides | ||||||
| TKRP-1 (x2) | APSGFLGLRa | 916,5363 | 916,5423 | 0,0435 | 100 | 100 |
| TKRP-3 | APSGFLGMRa | 934,4927 | 934,4969 | 0,0437 | 95 | 90 |
| TKRP-2 | VPSGFTGMRa | 950,4876 | 950,4980 | 0,0429 | 95 | 95 |
| TKRP-4 | VPNGFLGVRa | 957,5629 | 957,5361 | 0,0598 | 60 | 60 |
Putative Ae. aegypti neuropeptides detected by the direct profiling of single ALs, and matching with masses calculated from sequences of 28 predicted peptides stemming from 10 different neuropeptide genes in Ae. aegypti. Masses, which we obtained at most once per sex, were not included in further analysis (1770.8149 (sNPF-PP 4), 2229.9816 (sNPF-PP 2), 2599.2682 (NPLP-1–8)). x2: two copies on respective precursor (Predel et al., 2010).