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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Stem Cells. 2013 Jul;31(7):1350–1362. doi: 10.1002/stem.1382

Figure 7.

Figure 7

Sphere-derived colonies are multipotential. A. Sphere-derived colonies were subjected to differentiation protocols (Materials and Methods; Supplemental Methods) and three weeks later the cells were immunostained for the indicated lineage markers. Tubb3 and GFAP demonstrate ectodermal lineage, Myogenin and C-Troponin I mesodormal lineage, and CD31 and AFP endodermal lineage. B. As a control, no immunostaining was evident for any of the markers prior to differentiation. C. Differentiated colonies stained with oil red (adipose), alcian blue (cartilage) or alizarin red (calcified bone). D. Percentages of differentiated cell expressing each of the lineage markers. Standard deviations are shown. E. Model summarizing the pathway leading to reprogramming and immortalization of fibroblasts in spheres (shown as red circle). See text for discussion. The scale bar represents 20 μm.