Figure 4.

Low Levels of Neural Crest Transcripts Were Present in CHIR-Treated Cultures

hESCs were differentiated, harvested, and analyzed as described above for markers of (A) pluripotency and neural ectoderm markers, (B) differentiated neural and traditional cardiac markers, (C) specific markers of mature cardiomyocytes, and (D) patterned dermomyotome genes (n = 3 independent experiments, ^∗p ≤ 0.05 versus day 0, ^∗∗p ≤ 0.05 versus control). In (C) RNA from serum-differentiated hESCs and human heart (HH) cardiomyocyte-positive controls were obtained (n = 1). All hESC fold changes (black bars) were plotted on the primary y axis, and HH fold change (gray bar) was plotted on the secondary y axis.

Results are shown ±SEM.